NastyWoman
Senior Associate
Joined: Dec 24, 2010 20:50:37 GMT -5
Posts: 14,350
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Post by NastyWoman on Jul 18, 2020 18:04:42 GMT -5
Well I can't say I know what I'm talking about...... but I did look it up They are using IgG antibodies because they are associated with viral neutralising activity..... and they last longer than IgM or IgA (Don't ask any questions because I won't know) Moderna vaccine is looking promising...though they are talking about 2021 We also have a RNA vaccine in phase 2. Imperial College London... that would be next year also.
The first results from the Oxford vaccine are due out next week in the Lancet...From what I've heard its making antibodies and stimulating T cells.
They are vaccinating, then infecting healthy young volunteers in a final push. The Chinese are vaccinating their military although they haven't done phase 3. They are either going to be super humans.... or dead because it isn't safe. Plasma trials are undergoing studies and are still in progress.
The COVID virus binds predominately to mucosal tissues, and these are protected mostly by IgA, not IgG. The fluid you'll find these in is saliva, bronchial fluid, gastric lavage, crevicular fluid, tears and around the genital/urinary tract. So looking at neutralizing IgG antibody in blood is really useless when you really need the antibody at the site where both the virus gains entrance into the body and the specific tissue it attacks. I suspect that this is why the plasma trials have not been a slam dunk like they usually are for cases where the convalescent antibodies are protective. It is because you are not directing the bolus of the antibody at the source of attack. The T cells I am much less sure of as this was work I did at the very beginning and I remember staining for subsets of T cells that had been primed for a particular antigen. Then I could purify out that subset of cells and see what percentage of cells had been primed by the antigen. It is an incredibly laborious process, and I can't think of a way that this can really be done in bulk. I do know that they are involved in the production of secretory IgA antibodies, and that there is communication between the cell sets in producing a protective effect. Thank you for putting this in "plain English" so I can understand at a basic level. You just did what I force the engineers at my company (satellite mnfg) to do all the time, translate their gobbledygook into something I can work with. And if they want to change or add anything to their contract they'd better or I won't budge.
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anciana
Well-Known Member
Joined: Sept 20, 2011 11:34:57 GMT -5
Posts: 1,063
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Post by anciana on Jul 20, 2020 16:05:10 GMT -5
Seems like someone is looking into antibodies in breast milk for potential treatments. I wonder how much they have delved into the oral/dental health literature? We have been testing saliva successfully for secretory IgA antibody by ELISA since around 1980, and have published the tricks to it around that time. I think the last time I was looking at saliva antibody in humans and published on it was around 2006ish. I remember that study well, as we got some really interesting data from the African American population who smoked, and we had a horrible time those recruiting subjects. We finally did publish on it, where AA broke out significantly as a group even as < 10% of our subjects. I tried to recruit more to examine this, but had little luck. In fact, one of my first jobs was milking rats for secretory IgA. Breast milk has the highest concentration of sIgA, so when we needed to purify it for standards and to make antibodies to sIgA for ELISAs we had to make it from scratch.
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Post by The Walk of the Penguin Mich on Jul 20, 2020 16:17:07 GMT -5
Seems like someone is looking into antibodies in breast milk for potential treatments. I wonder how much they have delved into the oral/dental health literature? We have been testing saliva successfully for secretory IgA antibody by ELISA since around 1980, and have published the tricks to it around that time. I think the last time I was looking at saliva antibody in humans and published on it was around 2006ish. I remember that study well, as we got some really interesting data from the African American population who smoked, and we had a horrible time those recruiting subjects. We finally did publish on it, where AA broke out significantly as a group even as < 10% of our subjects. I tried to recruit more to examine this, but had little luck. In fact, one of my first jobs was milking rats for secretory IgA. Breast milk has the highest concentration of sIgA, so when we needed to purify it for standards and to make antibodies to sIgA for ELISAs we had to make it from scratch. Excellent! This makes far more sense than looking at IgG in plasma. Whoda thunk that the information I got milking rats would have such an application?
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